Home    About Us    Medic-All    Bio B2B    Hot Topic     Expo     Login | Join Now          
Bio B2BExcelsior Bio-System, Inc. 〉FLISA (IgE/IgG test)

FLISA (IgE/IgG test)

FLISA (IgE/IgG test)

Fluorescence-Linked Immunosorbent Array

Request for Collaboration
Overview
A. Microarray protein chip :
The foundation of the EBS microarray protein chip is based on using protein as probe markers which are spotted on the surface of a glass slide. To perform the test, serum sample is added to the test area creating an antigen-antibody binding reaction. A secondary antibody with fluorescent dye is then added to the surface allowing for specific wavelengths of the laser scanner to pin-point the specific antibodies induced by allergic reactions. Finally, three different concentrations of standard reagent are used to calculate the actual values and distributed over a standard curve to produce an accurate result.
 
B. Spotting system: BioDot AD3200 
Our allergen protein chip uses the BioDot AD3200 Spotting system. This system uses a programmable robotic arm with a three-dimensional axis and specialized slit needles (using the “Siphon Theory”) to transfer the allergens onto the spotting surface.
 
C. Scan system :
EBS Specific IgE/IgG Allergy Tests utilizes the Axon 4100B laser scanner. By using a specific wavelength, the florescence dye which is bounded to a secondary antibody is excited and thus producing a special signal. We can then use the AxSys software to analyze florescence signals to generate an easy to read report in a short amount of time.
 
Compared to the traditional ELISA (Enzyme-linked immune-sorbent assay) allergy analysis, EBS' allergy testing platform is systematicized by an innovative semi-automatic scanner and analysis software. The advantages of our platform are :
I. Minimal serum : Lower amount of serum sample required.
II. Stability and sensitivity : We conduct triplicate tests on each chip to ensure the stability of each batch. At the same time, we can detect the concentration limits (limitation can reach up to “pg” grade).
III. Environmentally-friendly and cost effective : Small amount of reagents and consumables are used to perform the test, therefore lowering the consumption of resources.
IV. High efficiency : Multiple samples can be conducted simultaneously and the results are available within 1~2 hours.
V. High flexibility : The FLISA platform allows for added flexibility of test items. As test items increase, the amount of serum sample and reagent can remain unchanged.
People who like this also like
  • Platform_BioSeizer™Platform_BioSeizer™
  • Breast Image Matching Method and Image Processing ApparatusBreast Image Matching Method and Image Processing Apparatus
  • Implant SystemImplant System
  • PHDC-01PHDC-01
  • PEP503 (A Radio-enhancer)PEP503 (A Radio-enhancer)
  • Medical Image Processing Apparatus and Breast Image Processing Method ThereofMedical Image Processing Apparatus and Breast Image Processing Method Thereof
  • Pilot scale up development and manufacturingPilot scale up development and manufacturing
  • JHL1266 Denosumab BiosimilarJHL1266 Denosumab Biosimilar
  • LT3001LT3001
  • TLC590TLC590